LOCUS       40924_26994        7570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKS145 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Terada Y, Park H-S., Kuroki Y, Fujiyama A.
  TITLE     Cloning vector pKS145
  JOURNAL   Published Only in Database (1998)
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Terada Y, Park H-S., Kuroki Y, Fujiyama A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1998) Contact:Asao Fujiyama National Institute of 
            Genetics, Human Genetics; Yata 1111, Mishima 411-8540, Japan
REFERENCE   3  (bases 1 to 7570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (1998)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1998) Contact:Asao Fujiyama National Institute of Genetics, 
            Human Genetics; Yata 1111, Mishima 411-8540, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Dec 22, 2008 this sequence version replaced AB013921.1.
FEATURES             Location/Qualifiers
     source          1..7570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..89)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..106)
                     /label=pKS145_Fwd
                     /note="pKS145_Fwd"
     regulatory      complement(881..922)
                     /note="contains the T4 gene 32 terminator and a portion of
                     the polylinker from the omega interposon; omega terminator"
                     /regulatory_class="terminator"
     CDS             940..1797
                     /label=AmpR
                     /note="beta-lactamase"
     misc_feature    complement(2640..3113)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3189..4157)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(4160..5332)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(5658..5908)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5914..6666)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(6757..6976)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    7396..7417
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7432..7462
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7470..7486
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7494..7510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7532..7550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccgcg gacgtcgtac gtcgcgaagc tttcgaacgc gtatgcatcg atcacgtgga
       61 tccggcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      121 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      181 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
      241 gttaatcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc cagaaaaccg
      301 aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt
      361 cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc
      421 aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag
      481 gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa
      541 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      601 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      661 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
      721 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      781 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
      841 atcgccgaag tatcgactca actatcagag gtagttggcg gctggggtga tatttgatgc
      901 gtcctgcatt cgtatgcggt cgattgaaaa aggaagagta tgagtattca acatttccgt
      961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1141 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     1201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     1501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1621 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1861 aaaaggatct aggtgaagat cctttttgat aatctcatgc gccgcttcgc ggcgcggctt
     1921 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     1981 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     2041 gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt
     2101 aaaatctagc gagggcttta ctaagctgat ccggtggatg accttttgaa tgacctttaa
     2161 tagattatat tactaattaa ttggggaccc tagaggtccc cttttttatt ttaaaaattt
     2221 tttcacaaaa cggtttacaa gcataaagct gatccccggg aattctcctc tcacatgcgg
     2281 cctccatcag gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc
     2341 ccgaagccgc agcgcgtgga ggagaaacga cgaaacgtga ccgggtgagg aggccgtcga
     2401 cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tgggtaacca
     2461 ggtattttgt ccacataacc gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat
     2521 ccacagcagg catacaaccg cacaccgagg ttactccgtt ctacaggtta cgacgacatg
     2581 tcaatacttg cccttgacag gcattgatgg aatcgtagtc tcacgctgat agtctgatcg
     2641 acaatacaag tgggaccgtg gtcccagacc gataatcaga ccgacaacac gagtgggatc
     2701 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata
     2761 atcagaccga cgatacgagt gggaccgtgg ttccagacta ataatcagac cgacgatacg
     2821 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccatggtccc
     2881 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc
     2941 gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac
     3001 cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat
     3061 tatcagaccg acgatacaag tggaacagtg ggcccagaga gaatattcag gccagttatg
     3121 ctttctggcc tgtaacaaag gacattaagt aaagacagat aaacgtagac taaaacgtgg
     3181 tcgcatcagg gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt ctctatacac
     3241 tcagttggaa cacgagacct gtccaggtta agcaccattt tatcgccctt atacaatact
     3301 gtcgctccag gagcaaactg atgtcgtgag cttaaactag ttcttgatgc agatgacgtt
     3361 ttaagcacag aagttaaaag agtgataact tcttcagctt caaatatcac cccagctttt
     3421 ttctgctcat gaaggttaga tgcctgctgc ttaagtaatt cctctttatc tgtaaaggct
     3481 ttttgaagtg catcacctga ccgggcagat agttcaccgg ggtgagaaaa aagagcaaca
     3541 actgatttag gcaatttggc ggtgttgata cagcgggtaa taatcttacg tgaaatattt
     3601 tccgcatcag ccagcgcaga aatatttcca gcaaattcat tctgcaatcg gcttgcataa
     3661 cgctgaccac gttcataagc acttgttggg cgataatcgt tacccaatct ggataatgca
     3721 gccatctgct catcatccag ctcgccaacc agaacacgat aatcactttc ggtaagtgca
     3781 gcagctttac gacggcgact cccatcggca atttctatga caccagatac tcttcgaccg
     3841 aacgccggtg tctgttgacc agtcagtaga aaagaaggga tgagatcatc cagtgcgtcc
     3901 tcagtaagca gctcctggtc acgttcatta cctgaccata cccgagaggt cttctcaaca
     3961 ctatcacccc ggagcacttc aagagtaaac ttcacatccc gaccacatac aggcaaagta
     4021 atggcattac cgcgagccat tactcctacg cgcgcaatta acgaatccac catcggggca
     4081 gctggtgtcg ataacgaagt atcttcaacc ggttgagtat tgagcgtatg ttttggaata
     4141 acaggcgcac gcttcattat ctaatctccc agcgtggttt aatcagacga tcgaaaattt
     4201 cattgcagac aggttcccaa atagaaagag catttctcca ggcaccagtt gaagagcgtt
     4261 gatcaatggc ctgttcaaaa acagttctca tccggatctg acctttacca acttcatccg
     4321 tttcacgtac aacatttttt agaaccatgc ttccccaggc atcccgaatt tgctcctcca
     4381 tccacgggga ctgagagcca ttactattgc tgtatttggt aagcaaaata cgtacatcag
     4441 gctcgaaccc tttaagatca acgttcttga gcagatcacg aagcatatcg aaaaactgca
     4501 gtgcggaggt gtagtcaaac aactcagcag gcgtgggaac aatcagcaca tcagcagcac
     4561 atacgacatt aatcgtgccg atacccaggt taggcgcgct gtcaataact atgacatcat
     4621 agtcatgagc aacagtttca atggccagtc ggagcatcag gtgtggatcg gtgggcagtt
     4681 taccttcatc aaatttgccc attaactcag tttcaatacg gtgcagagcc agacaggaag
     4741 gaataatgtc aagccccggc cagcaagtgg gctttattgc ataagtgaca tcgtcctttt
     4801 ccccaagata gaaaggcagg agagtgtctt ctgcatgaat atgaagatct ggtacccatc
     4861 cgtgatacat tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa acacgtagcc
     4921 ccttcagagc cagatcctga gcaagatgaa cagaaactga ggttttgtaa acgccacctt
     4981 tatgggcagc aaccccgatc accggtggaa atacgtcttc agcacgtcgc aatcgcgtac
     5041 caaacacatc acgcatatga ttaatttgtt caattgtata accaacacgt tgctcaaccc
     5101 gtcctcgaat ttccatatcc gggtgcggta gtcgccctgc tttctcggca tctctgatag
     5161 cctgagaaga aaccccaact aaatccgctg cttcacctat tctccagcgc cgggttattt
     5221 tcctcgcttc cgggctgtca tcattaaact gtgcaatggc gatagccttc gtcatttcat
     5281 gaccagcgtt tatgcactgg ttaagtgttt ccatgagttt cattctgaac atcctttaat
     5341 cattgctttg cgttttttta ttaaatcttg caatttactg caaagcaaca acaaaatcgc
     5401 aaagtcatca aaaaaccgca aagttgttta aaataagagc aacactacaa aaggagataa
     5461 gaagagcaca tacctcagtc acttattatc actagcgctc gccgcagccg tgtaaccgag
     5521 catagcgagc gaactggcga ggaagcaaag aagaactgtt ctgtcagata gctcttacgc
     5581 tcagcgcaag aagaaatatc caccgtggga aaaactccag gtagaggtac acacgcggat
     5641 agccaattca gagtaataaa ctgtgataat caaccctcat caatgatgac gaactaaccc
     5701 ccgatatcag gtcacatgac gaagggaaag agaaggaaat caactgtgac aaactgccct
     5761 caaatttggc ttccttaaaa attacagttc aaaaagtatg agaaaatcca tgcaggctga
     5821 aggaaacagc aaaactgtga caaattaccc tcagtaggtc agaacaaatg tgacgaacca
     5881 ccctcaaatc tgtgacagat aaccctcaga ctatcctgtc gtcatggaag tgatatcgcg
     5941 gaaggaaaat acgatatgag tcgtctggcg gcctttcttt ttctcaatgt atgagaggcg
     6001 cattggagtt ctgctgttga tctcattaac acagacctgc aggaagcggc ggcggaagtc
     6061 aggcatacgc tggtaacttt gaggcagctg gtaacgctct atgatccagt cgattttcag
     6121 agagacgatg cctgagccat ccggcttacg atactgacac agggattcgt ataaacgcat
     6181 ggcatacgga ttggtgattt cttttgtttc actaagccga aactgcgtaa accggttctg
     6241 taacccgata aagaagggaa tgagatatgg gttgatatgt acactgtaaa gccctctgga
     6301 tggactgtgc gcacgtttga taaaccaagg aaaagattca tagccttttt catcgccggc
     6361 atcctcttca gggcgataaa aaaccacttc cttccccgcg aaactcttca atgcctgccg
     6421 tatatcctta ctggcttccg cagaggtcaa tccgaatatt tcagcatatt tagcaacatg
     6481 gatctcgcag ataccgtcat gttcctgtag ggtgccatca gattttctga tctggtcaac
     6541 gaacagatac agcatacgtt tttgatcccg ggagagacta tatgccgcct cagtgaggtc
     6601 gtttgactgg acgattcgcg ggctattttt acgtttcttg tgattgataa ccgctgtttc
     6661 cgccatgaca gatccatgtg aagtgtgaca agtttttaga ttgtcacact aaataaaaaa
     6721 gagtcaataa gcagggataa ctttgtgaaa aaacagcttc ttctgagggc aatttgtcac
     6781 agggttaagg gcaatttgtc acagacagga ctgtcatttg agggtgattt gtcacactga
     6841 aagggcaatt tgtcacaaca ccttctctag aaccagcatg gataaaggcc tacaaggcgc
     6901 tctaaaaaag aagatctaaa aactataaaa aaaataatta taaaaatatc cccgtggata
     6961 agtggataac cccaagggaa gttttttcag gcatcgtgtg taagcagaat atataagtgc
     7021 tgttccctgg tgcttcctcg ctcactcgaa attcccgggg atagctttat gcttgtaaac
     7081 cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag
     7141 taatataatc tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc
     7201 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
     7261 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
     7321 cactgatccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7381 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7441 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7501 cagctatgac catgattacg ccaagcgcgc gtaatacgac tcactatagg gcggccgcta
     7561 gcatgctcga
//
